Regions of interest included abdominal total area, total fat area, subcutaneous fat area, visceral fat area (AVF), and right and left thigh total area, fat area, and skeletal muscle area. Adipose tissue and skeletal muscle cross-sectional areas (centimeters squared) were calculated using standard Hounsfield unit ranges (adipose tissue: -190 to -30 and skeletal muscle: -29 to 150). Two trained investigators analyzed each computed tomography image in duplicate. RESEARCH METHODS AND PROCEDURES: Abdominal and midthigh images were obtained using single-slice computed tomography.
Imagej software nih software#
OBJECTIVE: To compare reliability and limits of agreement of soft tissue cross-sectional areas obtained using Slice-O-Matic and NIH ImageJ medical imaging software packages.
DISCUSSION: These findings show that both the Slice-O-Matic and NIH ImageJ medical imaging software systems provide reliable measurements of adipose tissue and skeletal muscle cross-sectional areas.
The mean differences (95% confidence intervals) between the AVF cross-sectional areas obtained using the Slice-O-Matic and NIH ImageJ medical imaging software were +2.5 cm² (-5.7, +10.8 cm²) or +1.4% (-3.4%, +6.4%). Bland-Altman analyses revealed that Slice-O-Matic and NIH ImageJ results were comparable. Mean AVF values for investigators A and B ranged from 168 to 170 cm² using Slice-O-Matic and NIH ImageJ. RESULTS: For all images, intra-investigator coefficients of variation ranged from 0.2% to 3.4% and from 0.4% to 5.6% and inter-investigator coefficients of variation ranged from 0.9% to 4.8% and 0.2% to 2.6% for Slice-O-Matic and NIH ImageJ, respectively, with intra- and inter-investigator coefficients of reliability of R² = 0.99.